166 Determination of ß-Glucan in Barley, Oat and Rye

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Methods Type: Generic Methods
Key data
Number: 166
Analyte: ß-Glucan
Matrix: Barley, Oat and Rye
Year of Approval: 1998
Scope: This international standard specifies a method for the determination of ß-D-Glucan. This method is applicable to cereal grains (e.g. barley, oat, rye), their milling products and cereal based foods containing high level of glucose, after pre-extraction with aqueous ethanol.
Principle: ß-D-glucan is determined using highly purified lichenase and ß-D-glucosidase. ß-D-glucan is specifically hydrolyzed by lichenase to oligosaccharides, which are quantitatively cleaved to glucose by ß-glucosidase. Glucose is measured using glucose oxidase - peroxides - buffer mixture.

Method is rapid procedure for direct, quantitative measurement of (1->3) (1->4)-ß-D-glucan (ß-D-using highly purified lichenase and ß-glucosidase). ß-D-Glucan is specifically hydrolyzed by lichenase to oligosaccharides, which are then quantitatively cleaved to glucose by ß-glucosidase. Glucose is measured using glucose oxidase-peroxidase-buffer mixture.

Definition

This method determines quantitative (1->3), 1->4) - ß-D-glucan (ß-D-glucan, mixed-linkage ß-D-glucan).

 

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