Colorimetric Method for the Determination of Alpha-amylase Activity

Methods Type:

Generic Methods

Key data

Number:

108

Analyte:

Alpha-amylase Activity (Colorimetric Method)

Matrix:

Cereals or Cereal Products

Year of Approval:

1968

Scope:

The method is applicable to cereals or cereal products ranging from very low to very high in alpha-amylase activity. It can also be used for estimating the alpha-amylase activity of additives of fungal and bacterial origin.

Principle:

The decrease with time of the intensity of colour obtained with the diluted iodine solution is used as an index of starch degradation.

Definition

Alpha-amylase activity is expressed as a function of alpha-amylase concentration and of the velocity constant for the hydrolytic degradation of limit dextrin.

Colorimetric Method for the Determination of Alpha-amylase Activity

Definition

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